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3.
Membranes (Basel) ; 13(12)2023 Dec 14.
Artigo em Inglês | MEDLINE | ID: mdl-38132911

RESUMO

Dehiscence in surgeries involving membranes often leads to bacterial contamination, hindering the healing process. This study assessed bacterial colonization on various membrane materials. Polydioxanone (PDO) membranes, with thicknesses of 0.5 mm and 1 mm, and a collagen membrane were examined. Packages containing polystyrene pins were crafted using these membranes, attached to 24-well plates, and exposed to oral bacteria from supra and subgingival biofilm. After a week's anaerobic incubation, biofilm formation was evaluated using the DNA-DNA hybridization test. Statistical analysis employed the Kruskal-Wallis test with Dunn's post hoc test. The biofilm on the polystyrene pins covered by the 0.5 mm PDO membrane showed a higher count of certain pathogens. The collagen membrane had a greater total biofilm count on its inner surface compared to both PDO membranes. The external collagen membrane face had a higher total biofilm count than the 0.5 mm PDO membrane. Furthermore, the 1 mm PDO membrane exhibited a greater count of specific pathogens than its 0.5 mm counterpart. In conclusion, the collagen membrane presented more biofilm and pathogens both internally and on its inner surface.

4.
Almeida, André Luiz Cerqueira; Melo, Marcelo Dantas Tavares de; Bihan, David Costa de Souza Le; Vieira, Marcelo Luiz Campos; Pena, José Luiz Barros; Del Castillo, José Maria; Abensur, Henry; Hortegal, Renato de Aguiar; Otto, Maria Estefania Bosco; Piveta, Rafael Bonafim; Dantas, Maria Rosa; Assef, Jorge Eduardo; Beck, Adenalva Lima de Souza; Santo, Thais Harada Campos Espirito; Silva, Tonnison de Oliveira; Salemi, Vera Maria Cury; Rocon, Camila; Lima, Márcio Silva Miguel; Barberato, Silvio Henrique; Rodrigues, Ana Clara; Rabschkowisky, Arnaldo; Frota, Daniela do Carmo Rassi; Gripp, Eliza de Almeida; Barretto, Rodrigo Bellio de Mattos; Silva, Sandra Marques e; Cauduro, Sanderson Antonio; Pinheiro, Aurélio Carvalho; Araujo, Salustiano Pereira de; Tressino, Cintia Galhardo; Silva, Carlos Eduardo Suaide; Monaco, Claudia Gianini; Paiva, Marcelo Goulart; Fisher, Cláudio Henrique; Alves, Marco Stephan Lofrano; Grau, Cláudia R. Pinheiro de Castro; Santos, Maria Veronica Camara dos; Guimarães, Isabel Cristina Britto; Morhy, Samira Saady; Leal, Gabriela Nunes; Soares, Andressa Mussi; Cruz, Cecilia Beatriz Bittencourt Viana; Guimarães Filho, Fabio Villaça; Assunção, Bruna Morhy Borges Leal; Fernandes, Rafael Modesto; Saraiva, Roberto Magalhães; Tsutsui, Jeane Mike; Soares, Fábio Luis de Jesus; Falcão, Sandra Nívea dos Reis Saraiva; Hotta, Viviane Tiemi; Armstrong, Anderson da Costa; Hygidio, Daniel de Andrade; Miglioranza, Marcelo Haertel; Camarozano, Ana Cristina; Lopes, Marly Maria Uellendahl; Cerci, Rodrigo Julio; Siqueira, Maria Eduarda Menezes de; Torreão, Jorge Andion; Rochitte, Carlos Eduardo; Felix, Alex.
Arq. bras. cardiol ; 120(12): e20230646, dez. 2023. tab, graf
Artigo em Português | LILACS-Express | LILACS, SESSP-IDPCPROD, Sec. Est. Saúde SP | ID: biblio-1527794
5.
Nat Nanotechnol ; 2023 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-38012274

RESUMO

Mutations in SARS-CoV-2 have shown effective evasion of population immunity and increased affinity to the cellular receptor angiotensin-converting enzyme 2 (ACE2). However, in the dynamic environment of the respiratory tract, forces act on the binding partners, which raises the question of whether not only affinity but also force stability of the SARS-CoV-2-ACE2 interaction might be a selection factor for mutations. Using magnetic tweezers, we investigate the impact of amino acid substitutions in variants of concern (Alpha, Beta, Gamma and Delta) and on force-stability and bond kinetic of the receptor-binding domain-ACE2 interface at a single-molecule resolution. We find a higher affinity for all of the variants of concern (>fivefold) compared with the wild type. In contrast, Alpha is the only variant of concern that shows higher force stability (by 17%) compared with the wild type. Using molecular dynamics simulations, we rationalize the mechanistic molecular origins of this increase in force stability. Our study emphasizes the diversity of contributions to the transmissibility of variants and establishes force stability as one of the several factors for fitness. Understanding fitness advantages opens the possibility for the prediction of probable mutations, allowing a rapid adjustment of therapeutics, vaccines and intervention measures.

6.
Mar Pollut Bull ; 197: 115717, 2023 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-37924737

RESUMO

The presence of marine litter in the deep ocean (>200 m depth) may represent an invisible threat that has been neglected due to the scarcity of data. Herein, we provide the first report of persistent marine litter on the Southwestern Atlantic continental slope. Marine litter was collected onboard the Brazilian R/V Alpha Crucis, using bottom trawling, in 28 out of 31 sampled stations, between 274 and 1520 m depth, in two distinct areas off the southern Brazilian coast. In total, 603 items and 13.8 kg of litter were collected and classified according to the type of material. Plastic was the most frequent and most abundant material found. Although there was no bathymetric variation along the slope, density of litter was considerably higher off São Paulo than off Santa Catarina State, supposedly due to the heavier presence of oil and gas platforms and large cargo vessels.


Assuntos
Monitoramento Ambiental , Plásticos , Brasil , Resíduos/análise
7.
PLoS Negl Trop Dis ; 17(11): e0011762, 2023 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-38019886

RESUMO

INTRODUCTION: Zika virus infection during pregnancy causes fetal microcephaly and brain damage. Congenital Zika syndrome (CZS) is characterized by systemic involvement with diffuse muscle impairment, a high frequency of arthrogryposis, and microphthalmia. Cardiac impairment in CZS has rarely been evaluated. Our study assessed morphology and biventricular cardiac function in children with CZS and advanced neurological dysfunction. METHODS: This cross-sectional study was conducted on 52 children with CZS (Zika group; ZG) and 25 healthy children (control group; CG) in Paraiba, Brazil. Clinical evaluation, electrocardiogram (EKG), and transthoracic echocardiogram (TTE) were performed on all children. Additionally, troponin I and natriuretic peptide type B (BNP) levels, the degree of cerebral palsy, and neuroimaging findings were assessed in the ZG group. RESULTS: The median age of the study population was 5 years in both groups, and 40.4% (ZG) and 60% (CG) were female. The most prevalent electrocardiographic alteration was sinus arrhythmia in both the ZG (n = 9, 17.3%) and CG (n = 4, 16%). The morphological parameters adjusted for Z score were as follows: left ventricular (LV) end-diastolic diameter in ZG: -2.36 [-5.10, 2.63] vs. CG: -1.07 [-3.43, 0.61], p<0.001); ascending aorta (ZG: -0.09 [-2.08, 1.60] vs. CG: 0.43 [-1.47, 2.2], p = 0.021); basal diameter of the right ventricle (RV) (ZG: -2.34 [-4.90, 0.97] vs. CG: -0.96 [-2.21, 0.40], p<0.01); and pulmonary artery dimension (ZG: -2.13 [-5.99, 0.98] vs. CG: -0.24 [-2.53, 0.59], p<0.01). The ejection fractions (%) were 65.7 and 65.6 in the ZG and CG, respectively (p = 0.968). The left atrium volume indices (mL/m2) in the ZG and CG were 13.15 [6.80, 18.00] and 18.80 [5.90, 25.30] (p<0.01), respectively, and the right atrium volume indices (mL/m2) were 10.10 [4.90, 15.30] and 15.80 [4.10, 24.80] (p<0.01). The functional findings adjusted for Z score were as follows: lateral systolic excursion of the mitral annular plane (MAPSE) (ZG: 0.36 [-2.79, 4.71] vs. CG: 1.79 [-0.93, 4.5], p = 0.001); tricuspid annular plane systolic excursion (TAPSE) (ZG: -2.43 [-5.47, 5.09] vs. CG: 0.07 [-1.98, 3.64], p<0.001); and the S' of the RV (ZG: 1.20 [3.35, 2.90] vs. CG: -0.20 [-2.15, 1.50], p = 0.0121). No differences in biventricular strain measurements were observed between the groups. Troponin I and BNP levels were normal in in the ZG. Grade V cerebral palsy and subcortical calcification were found in 88.6% and 97.22% of children in the ZG group, respectively. CONCLUSION: A reduction in cardiac dimensions and functional changes were found in CZS patients, based on the TAPSE, S' of the RV, and MAPSE, suggesting the importance of cardiac evaluation and follow-up in this group of patients.


Assuntos
Paralisia Cerebral , Infecção por Zika virus , Zika virus , Criança , Humanos , Feminino , Pré-Escolar , Masculino , Infecção por Zika virus/complicações , Estudos Transversais , Troponina I , Ecocardiografia
8.
Cell Host Microbe ; 31(8): 1260-1274.e6, 2023 08 09.
Artigo em Inglês | MEDLINE | ID: mdl-37516110

RESUMO

Molecular de-extinction could offer avenues for drug discovery by reintroducing bioactive molecules that are no longer encoded by extant organisms. To prospect for antimicrobial peptides encrypted within extinct and extant human proteins, we introduce the panCleave random forest model for proteome-wide cleavage site prediction. Our model outperformed multiple protease-specific cleavage site classifiers for three modern human caspases, despite its pan-protease design. Antimicrobial activity was observed in vitro for modern and archaic protein fragments identified with panCleave. Lead peptides showed resistance to proteolysis and exhibited variable membrane permeabilization. Additionally, representative modern and archaic protein fragments showed anti-infective efficacy against A. baumannii in both a skin abscess infection model and a preclinical murine thigh infection model. These results suggest that machine-learning-based encrypted peptide prospection can identify stable, nontoxic peptide antibiotics. Moreover, we establish molecular de-extinction through paleoproteome mining as a framework for antibacterial drug discovery.


Assuntos
Anti-Infecciosos , Peptídeos Antimicrobianos , Animais , Humanos , Camundongos , Peptídeos/farmacologia , Antibacterianos/farmacologia , Aprendizado de Máquina , Peptídeo Hidrolases , Testes de Sensibilidade Microbiana
9.
Artigo em Inglês | MEDLINE | ID: mdl-37297537

RESUMO

Heart failure is a prevalent condition and a frequent cause of hospital readmissions and poor quality of life. Teleconsultation support from cardiologists to primary care physicians managing patients with heart failure may improve care, but the effect on patient-relevant outcomes is unclear. We aim to evaluate whether collaboration through a novel teleconsultation platform in the Brazilian Heart Insufficiency with Telemedicine (BRAHIT) project, tested on a previous feasibility study, can improve patient-relevant outcomes. We will conduct a parallel-group, two-arm, cluster-randomised superiority trial with a 1:1 allocation ratio, with primary care practices from Rio de Janeiro as clusters. Physicians from the intervention group practices will receive teleconsultation support from a cardiologist to assist patients discharged from hospitals after admission for heart failure. In contrast, physicians from the control group practices will perform usual care. We will include 10 patients per each of the 80 enrolled practices (n = 800). The primary outcome will be a composite of mortality and hospital admissions after six months. Secondary outcomes will be adverse events, symptoms frequency, quality of life, and primary care physicians' compliance with treatment guidelines. We hypothesise that teleconsulting support will improve patient outcomes.


Assuntos
Insuficiência Cardíaca , Telemedicina , Humanos , Qualidade de Vida , Brasil , Telemedicina/métodos , Insuficiência Cardíaca/terapia , Atenção Primária à Saúde , Ensaios Clínicos Controlados Aleatórios como Assunto
10.
ABC., imagem cardiovasc ; 36(1): e371, abr. 2023. ilus
Artigo em Português | LILACS | ID: biblio-1513116

RESUMO

Fundamento: A avaliação da área valvar mitral por meio da reconstrução multiplano na ecocardiografia tridimensional é restrita a softwares específicos e à experiência dos ecocardiografistas. Eles precisam selecionar manualmente o frame do vídeo que contenha a área de abertura máxima da valva mitral, dimensão fundamental para a identificação de estenose mitral. Objetivo: Automatizar o processo de determinação da área de abertura máxima da valva mitral, por meio da aplicação de Processamento Digital de Imagens (PDI) em exames de ecocardiograma, desenvolvendo um algoritmo aberto com leitura de vídeo no formato avi. Método: Este estudo piloto observacional transversal foi realizado com vinte e cinco exames diferentes de ecocardiograma, sendo quinze com abertura normal e dez com estenose mitral reumática. Todos os exames foram realizados e disponibilizados por dois especialistas, com autorização do Comitê de Ética em Pesquisa, que utilizaram dois modelos de aparelhos ecocardiográficos: Vivid E95 (GE Healthcare) e Epiq 7 (Philips), com sondas multiplanares transesofágicas. Todos os vídeos em formato avi foram submetidos ao PDI através da técnica de segmentação de imagens. Resultados: As medidas obtidas manualmente por ecocardiografistas experientes e os valores calculados pelo sistema desenvolvido foram comparados utilizando o diagrama de Bland-Altman. Observou-se maior concordância entre valores no intervalo de 0,4 a 2,7 cm². Conclusão: Foi possível determinar automaticamente a área de máxima abertura das valvas mitrais, tanto para os casos advindos da GE quanto da Philips, utilizando apenas um vídeo como dado de entrada. O algoritmo demonstrou economizar tempo nas medições quando comparado com a mensuração habitual. (AU)


Background: The evaluation of mitral valve area through multiplanar reconstruction in 3-dimensional echocardiography is restricted to specific software and to the experience of echocardiographers. They need to manually select the video frame that contains the maximum mitral valve opening area, as this dimension is fundamental to identification of mitral stenosis. Objective: To automate the process of determining the maximum mitral valve opening area, through the application of digital image processing (DIP) in echocardiography tests, developing an open algorithm with video reading in avi format. Method: This cross-sectional observational pilot study was conducted with 25 different echocardiography exams, 15 with normal aperture and 10 with rheumatic mitral stenosis. With the authorization of the Research Ethics Committee, all exams were performed and made available by 2 specialists who used 2 models of echocardiographic devices: Vivid E95 (GE Healthcare) and Epiq 7 (Philips), with multiplanar transesophageal probes. All videos in avi format were submitted to DIP using the image segmentation technique. Results: The measurements obtained manually by experienced echocardiographers and the values calculated by the developed system were compared using a Bland-Altman diagram. There was greater agreement between values in the range from 0.4 to 2.7 cm². Conclusion: It was possible to automatically determine the maximum mitral valve opening area, for cases from both GE and Philips, using only 1 video as input data. The algorithm has been demonstrated to save time on measurements when compared to the usual method. (AU)


Assuntos
Humanos , Doenças das Valvas Cardíacas/mortalidade , Valva Mitral/fisiopatologia , Valva Mitral/diagnóstico por imagem , Estenose da Valva Mitral/etiologia , Processamento de Imagem Assistida por Computador/métodos , Doxorrubicina/efeitos da radiação , Ecocardiografia Transesofagiana/métodos , Ecocardiografia Tridimensional/métodos , Substituição da Valva Aórtica Transcateter/métodos , Isoproterenol/efeitos da radiação , Valva Mitral/cirurgia
11.
Acta Cir Bras ; 38: e380623, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36888756

RESUMO

PURPOSE: To evaluate the in vivo efficiency of commercial polymeric membranes for guided bone regeneration. METHODS: Rat calvarial critical size defects was treated with LuminaCoat (LC), Surgitime PTFE (SP), GenDerm (GD), Pratix (PR), Techgraft (TG) or control (C-) and histomorphometric analysis determined the percentage of new bone, connective tissue and biomaterial at 1 or 3 months. Statistical analysis used ANOVA with Tukey's post-test for means at same experimental time and the paired Student's t test between the two periods, considering p < 0.05. RESULTS: New bone at 1 month was higher for SP, TG and C-, at 3 months there were no differences, and between 1 and 3 months PR had greater increase growthing. Connective tissue at 1 month was higher for C-, at 3 months for PR, TG and C-, and between 1 and 3 months C- had sharp decline. Biomaterial at 1 month was higher for LC, in 3 months for SP and TG, and between 1 and 3 months, LC, GD and TG had more decreasing mean. CONCLUSIONS: SP had greater osteopromotive capacity and limitation of connective ingrowth, but did not exhibit degradation. PR and TG had favorable osteopromotion, LC less connective tissue and GD more accelerated biodegradation.


Assuntos
Materiais Biocompatíveis , Regeneração Óssea , Ratos , Animais
12.
Biophys J ; 122(14): 2833-2840, 2023 07 25.
Artigo em Inglês | MEDLINE | ID: mdl-36738105

RESUMO

Over a century ago, physicists started broadly relying on theoretical models to guide new experiments. Soon thereafter, chemists began doing the same. Now, biological research enters a new era when experiment and theory walk hand in hand. Novel software and specialized hardware became essential to understand experimental data and propose new models. In fact, current petascale computing resources already allow researchers to reach unprecedented levels of simulation throughput to connect in silico and in vitro experiments. The reduction in cost and improved access allowed a large number of research groups to adopt supercomputing resources and techniques. Here, we outline how large-scale computing has evolved to expand decades-old research, spark new research efforts, and continuously connect simulation and observation. For instance, multiple publicly and privately funded groups have dedicated extensive resources to develop artificial intelligence tools for computational biophysics, from accelerating quantum chemistry calculations to proposing protein structure models. Moreover, advances in computer hardware have accelerated data processing from single-molecule experimental observations and simulations of chemical reactions occurring throughout entire cells. The combination of software and hardware has opened the way for exascale computing and the production of the first public exascale supercomputer, Frontier, inaugurated by the Oak Ridge National Laboratory in 2022. Ultimately, the popularization and development of computational techniques and the training of researchers to use them will only accelerate the diversification of tools and learning resources for future generations.


Assuntos
Inteligência Artificial , Software , Metodologias Computacionais , Simulação por Computador , Computadores
13.
J Bras Pneumol ; 49(1): e20220093, 2023.
Artigo em Inglês, Português | MEDLINE | ID: mdl-36790283

RESUMO

OBJECTIVE: To assess the influence of the COVID-19 pandemic on hospital admissions (HA), intra-hospital deaths (HD), and intra-hospital lethality rates (HL) related to respiratory diseases (RD) other than COVID-19 in Brazil. METHODS: This observational time-series study was conducted through comparative analyses of the HA, HD, and HL related to non-COVID-19 RD registered between March and December 2020 by the Brazilian Unified Public Health System on the DataSUS Tabnet platform, using as reference the values recorded in the same period of 2019 and those projected by linear regression methods for 2020, considering the period from 2015 to 2019. The adopted statistical significance level was 5% (p < 0.05). RESULTS: Compared to 2019, in 2020, there was a 42% decrease in HA and a 7.4% decrease in total HD related to non-COVID-19 RD, followed by a 60% increase in HL associated with this group of diseases. The HA and HL registered in 2020 differed significantly from the projected trend for that year by linear regression (p < 0.05). Of note, a significant reduction in hospitalizations due to asthma (-46%), chronic obstructive pulmonary disease (-45%), bronchiectasis (-54%), pneumonia (-46%), and acute bronchitis (-73%) was observed. CONCLUSIONS: During the first 8 months of the pandemic, there was a decline in HA and an increase in HL related to non-COVID-19 RD in Brazil, which can hypothetically reflect logistical challenges and delays in the management of this group of diseases.


Assuntos
COVID-19 , Transtornos Respiratórios , Doenças Respiratórias , Humanos , Pandemias , Brasil/epidemiologia , Saúde Pública , Hospitalização , Hospitais
14.
Pensar Prát. (Online) ; 26Fev. 2023. Ilus
Artigo em Português | LILACS | ID: biblio-1531696

RESUMO

Este estudo tem como objetivo analisar as contribuições teórico-metodológicas do jogo eletrônico para a formação de professores nos cursos de Licenciatura em Educação Física. Metodologicamente, optamos pela Hermenêutica-Dialética como método, que tem como princípio a comunicação (tratada neste estudo por meio de uma abordagem qualitativa com inserção no campo). Foi utilizada a coleta de dados, como o instrumento questionário, aplicado aos professores que tratam o conhecimento "jogo" na formação inicial em Educação Física, em uma Universidade pública. Concluímos que as contribuições teórico-metodológicas do jogo eletrônico para a formação de professores de Educação Física podem ser compreendidas em três tópicos: cultura, transposição da realidade virtual para a concreta e exergame (AU).


The purpose of this study was to analyze the theoretical and methodological contributions of electronic games to teachers training for a Physical Education Licentiate degree. As for the methodology, we chose the hermeneutics-dialectic method, which views communication as a principle and is treated in this study through a qualitative approach inserted in the field. A questionnaire for data collection was applied to teachers who dealt with game knowledge in the initial formation of Physical Education at a public university. It was concluded that the theoretical-methodological contributions of electronic games for the formation of Physical Education teachers can be understood by three topics: culture, transposition of virtual reality into concrete reality, and exergame (AU).


Este estudio tiene como objetivo analizar los aportes teóricos y metodológicos de los juegos electrónicos a la formación de profesores en los cursos de Licenciatura en Educación Física. Metodológicamente, se optó por el método hermenéutico-dialéctico, que tiene como principio la comunicación, tratado en este estudio a través de un enfoque cualitativo con inserción en el campo, utilizando el instrumento cuestionario para la recolección de datos aplicado a los docentes que abordan el conocimiento del juego en la formación en Educación Física en una universidad pública. Concluimos que los aportes teórico-metodológicos de los juegos electrónicos para la formación de estos docentes se pueden entender en tres temas: cultura, transposición de la realidad virtual al concreto y exergame (AU).


Assuntos
Humanos , Capacitação de Professores , Tecnologia Digital , Jogos Eletrônicos de Movimento
15.
Licere (Online) ; 26(04): 168-197, dez.2023. ilus
Artigo em Português | LILACS-Express | LILACS | ID: biblio-1531459

RESUMO

Este trabalho analisou contribuições teórico-metodológicas da ludicidade com idosas institucionalizadas. Para isso, contou com a Hermenêutica-Dialética como método, que busca na compreensão de falas, texto e contexto, a concepção dos fenômenos estudados, os quais foram evidenciados pela entrevista semiestruturada com 19 (dezenove)participantes envolvidos num programa de extensão com intervenção em Instituição de Longa Permanência para Idosos (ILPI) da cidade do Recife. As falas tratadas pela análise de conteúdo categorial por temática revelaram duas categorias empíricas, das quais, optamos pela ludicidade. Como conclusão, foram evidenciados princípios lúdicos, a saber: alegria, diversão, gratuidade, imaginação, plenitude, liberdade e cultura, sendo facetas que fundamentam a prática lúdica num viés teórico-metodológico, revelando contribuições para a melhoria da interação social em ILPI.


This work analyzed the theoretical-methodological contributions of ludic activities with institutionalized elderly women. To this end, it was based on the Hermeneutics-Dialectics as a method, which pursues understanding in speeches, text and context, the conception of the phenomena studied, these were evidenced through a semi-structured interview with 19 participants who were involved in an extension program with intervention in a Long-Stay institution for the Elderly (LSIE), in the city of Recife. The speeches dealt with categorical content analysis by theme and revealed two empirical categories, from which, we opted for playfulness. In conclusion, ludic principles were evidenced, namely: joy, fun, gratuity, imagination, fullness, freedom and culture, these being facets that base the ludic practice on a theoretical-methodological bias, thus revealing contributions to the improvement of social interaction in LSIE.

16.
J Am Chem Soc ; 145(1): 70-77, 2023 01 11.
Artigo em Inglês | MEDLINE | ID: mdl-36455202

RESUMO

The unbinding pathway of a protein complex can vary significantly depending on biochemical and mechanical factors. Under mechanical stress, a complex may dissociate through a mechanism different from that used in simple thermal dissociation, leading to different dissociation rates under shear force and thermal dissociation. This is a well-known phenomenon studied in biomechanics whose molecular and atomic details are still elusive. A particularly interesting case is the complex formed by bacterial adhesins with their human peptide target. These protein interactions have a force resilience equivalent to those of covalent bonds, an order of magnitude stronger than the widely used streptavidin:biotin complex, while having an ordinary affinity, much lower than that of streptavidin:biotin. Here, in an in silico single-molecule force spectroscopy approach, we use molecular dynamics simulations to investigate the dissociation mechanism of adhesin/peptide complexes. We show how the Staphylococcus epidermidis adhesin SdrG uses a catch-bond mechanism to increase complex stability with increasing mechanical stress. While allowing for thermal dissociation in a low-force regime, an entirely different mechanical dissociation path emerges in a high-force regime, revealing an intricate mechanism that does not depend on the peptide's amino acid sequence. Using a dynamic network analysis approach, we identified key amino acid contacts that describe the mechanics of this complex, revealing differences in dynamics that hinder thermal dissociation and establish the mechanical dissociation path. We then validate the information content of the selected amino acid contacts using their dynamics to successfully predict the rupture forces for this complex through a machine learning model.


Assuntos
Infecções Bacterianas , Biotina , Humanos , Estreptavidina/química , Biotina/química , Ligação Proteica , Aminoácidos/metabolismo , Microscopia de Força Atômica
17.
Acta cir. bras ; 38: e380623, 2023. tab, graf, ilus
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1419861

RESUMO

Purpose: To evaluate the in vivo efficiency of commercial polymeric membranes for guided bone regeneration. Methods: Rat calvarial critical size defects was treated with LuminaCoat (LC), Surgitime PTFE (SP), GenDerm (GD), Pratix (PR), Techgraft (TG) or control (C-) and histomorphometric analysis determined the percentage of new bone, connective tissue and biomaterial at 1 or 3 months. Statistical analysis used ANOVA with Tukey's post-test for means at same experimental time and the paired Student's t test between the two periods, considering p < 0.05. Results: New bone at 1 month was higher for SP, TG and C-, at 3 months there were no differences, and between 1 and 3 months PR had greater increase growthing. Connective tissue at 1 month was higher for C-, at 3 months for PR, TG and C-, and between 1 and 3 months C- had sharp decline. Biomaterial at 1 month was higher for LC, in 3 months for SP and TG, and between 1 and 3 months, LC, GD and TG had more decreasing mean. Conclusion: SP had greater osteopromotive capacity and limitation of connective ingrowth, but did not exhibit degradation. PR and TG had favorable osteopromotion, LC less connective tissue and GD more accelerated biodegradation.


Assuntos
Animais , Ratos , Polímeros/uso terapêutico , Crânio/anormalidades , Materiais Biocompatíveis/análise , Regeneração Óssea , Colágeno , Regeneração Tecidual Guiada/veterinária
18.
Arq Bras Cardiol ; 120(12): e20230646, 2023 Dec.
Artigo em Português, Inglês | MEDLINE | ID: mdl-38232246

RESUMO

Central Illustration : Position Statement on the Use of Myocardial Strain in Cardiology Routines by the Brazilian Society of Cardiology's Department Of Cardiovascular Imaging - 2023 Proposal for including strain in the integrated diastolic function assessment algorithm, adapted from Nagueh et al.67 Am: mitral A-wave duration; Ap: reverse pulmonary A-wave duration; DD: diastolic dysfunction; LA: left atrium; LASr: LA strain reserve; LVGLS: left ventricular global longitudinal strain; TI: tricuspid insufficiency. Confirm concentric remodeling with LVGLS. In LVEF, mitral E wave deceleration time < 160 ms and pulmonary S-wave < D-wave are also parameters of increased filling pressure. This algorithm does not apply to patients with atrial fibrillation (AF), mitral annulus calcification, > mild mitral valve disease, left bundle branch block, paced rhythm, prosthetic valves, or severe primary pulmonary hypertension.


Figura Central : Posicionamento do Departamento de Imagem Cardiovascular da Sociedade Brasileira de Cardiologia sobre o Uso do Strain Miocárdico na Rotina do Cardiologista ­ 2023 Proposta de inclusão do strain no algoritmo integrado de avaliação da função diastólica, adaptado e traduzido de Nagueh et al. 67 AE: átrio esquerdo; Ap: duração da onda A reversa pulmonar; Am: duração da onda A mitral; DD: disfunção diastólica; FEVEr: fração de ejeção do ventrículo esquerdo reduzida; IT: insuficiência tricúspide; SAEr: strain do AE de reservatório; SLGVE: strain longitudinal global do ventrículo esquerdo. Se remodelamento concêntrico, confirmar com SLGVE. Na presença de FEVEr, tempo de desaceleração da onda E mitral (TDE) < 160 ms e onda S < D pulmonar também são parâmetros de pressão de enchimento aumentada. Esse algoritmo não se aplica a pacientes com fibrilação atrial (FA), calcificação do anel mitral ou valvopatia mitral maior que discreta, bloqueio de ramo esquerdo (BRE), ritmo de marca-passo, próteses valvares ou hipertensão pulmonar (HP) primária grave.


Assuntos
Fibrilação Atrial , Cardiologia , Disfunção Ventricular Esquerda , Humanos , Ecocardiografia Doppler , Brasil , Fibrilação Atrial/diagnóstico por imagem , Átrios do Coração/diagnóstico por imagem , Função Ventricular Esquerda
19.
Motrivivência (Florianópolis) ; 35(66): 1-19, 2023.
Artigo em Português | LILACS-Express | LILACS | ID: biblio-1451808

RESUMO

Presente no sistema universitário em âmbito federal para civis desde 1939, a formação em Educação Física conviveu com outros espaços de formação por longo tempo. Um desses espaços consiste nos cursos técnicos de habilitação de professores em Educação Física de 2° grau (atual ensino médio). Nesse sentido, que papel teria o curso técnico de formação de professores em Educação Física? Para responder tal questão, a pesquisa teve como objetivo específico analisar os cursos técnicos de 2° grau de formação de professores em Educação Física e a sua mediação com a formação profissional nas décadas de 1970, 1980 e 1990. Como material empírico foi realizada a análise documental de matrizes curriculares, portarias governamentais e legislações federais e estaduais (Rio de Janeiro) sobre educação, além da visita a quatro instituições de educação básica no Estado do Rio de Janeiro que, em outros tempos, ofertavam o referido curso.


Present in the university system at the federal level since 1939, it has lived with other spaces of formation for a long time. One of these spaces consists of the technical courses of habilitation of teachers in Physical Education at the level of 2nd degree (current high school). In this sense, what role would the technical course of teacher training in Physical Education have? In order to answer this question, the research had the specific objective of analyzing the technical courses of 2nd degree of teacher training in Physical Education and its mediation with professional training in the 1970s, 1980s and 1990s. As empirical material the analysis was performed documentary of curricular matrices, governmental ordinances and federal and state legislations (Rio de Janeiro) on education, besides the visitto four institutions of basic education in the Stateof Rio de Janeiro that offered the referred course at the time.


Presente en el sistema universitario a nivel federal para civiles desde 1939, convivió durante mucho tiempo com otros espacios de formación. Uno de estos espacios consiste en cursos técnicos para La formación de profesores de Educación Física en Secundaria. En este sentido, ¿qué papel tendría el curso técnico para La formación de profesores de Educación Física? Para responder a esta interrogante, La investigación tuvo como objetivo específico analizar los cursos técnicos de 2° grado de formación de profesores de Educación Física y su mediación com La formación profesional en las décadas de 1970, 1980 y 1990. Como material empírico, El análisis fue documental de matrices curriculares, ordenanzas gubernamentales y legislación federal y estatal (Río de Janeiro) sobre educación, además de la visita a cuatro instituciones de educación básica del Estado de Río de Janeiro que, em otros tiempos, ofrecieron este curso.

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